Thursday, May 16, 2024 | 1 PM–5 PM ET
Residence Inn Boston Cambridge
120 Broadway, Cambridge MA 02142 (Kendall Square)
“DOC” Edgerton Room
Join us for a FREE networking & learning event with PAT industry pros, end users, and partners.
View our event terms & conditions
PhD Candidate, MIT
Principal Engineer, Biogen
Lead Scientist, Tornado, a Bruker Company
Network with local industry professionals, end users and partners.
Get hands-on interaction with technology from Aber, Tornado, a Bruker Company, Securecell and ProAnalytics.
Included hors d’oeuvres and drinks with an opportunity to mingle with your peers.
Join us for this half-day event to network with industry professionals, end users, and partners at this exciting symposium near Kendall Square in Cambridge, MA.
This event and setting provide a unique opportunity to engage closely with leading experts who will share cutting-edge insights and present compelling case studies designed to facilitate collaborative discussions and exchanges of ideas.
There will also be hors d’oeuvres and drinks served with an opportunity to mingle with each other and interact “hands-on” with the equipment!
View our event terms & conditions
Aber Instruments is bringing Optura and Futura instrumentation to support biomass monitoring. Tornado (a Bruker Company) will have their HyperFlux PRO Plus and several Raman probes on hand. ProAnalytics will showcase Securecell’s Numera autosampler alongside their bioprocess software, Lucullus.
Biologic products, including mAbs, are structurally complex with several critical quality attributes (CQAs) that can affect immunogenicity, product stability, and clearance rates from the body. These product CQAs are directly related to processing conditions, and thus control of the process conditions can enable control of the CQAs. Unfortunately, the relationships between these process conditions and CQAs can require complex models to describe, which cannot be encoded in a traditional bioreactor control system. This presentation describes our work to control one such CQA, the glycosylation of a mAb, using model predictive control. We describe the software and hardware that has enabled us, including the REST API, which allows for communication between our Python-based model of the glycosylation process and Lucullus, our bioreactor control software.
Alexis Dubs – PhD Candidate, MIT
Various PAT technologies have been emerging over the past several decades to enhance the process productivity and robustness in biopharmaceutical manufacturing. However, their adoption hurdles have remained in the highly regulated bioprocesses. In this case study, we demonstrated a roadmap of developing an on-line monitoring and control of cell culture process using dielectric spectroscopy from lab scale to GMP manufacturing.
An Zhang – Principal Engineer, Biogen
Raman spectroscopy is an important Process Analytical technology (PAT) tool in the realm of biopharmaceutical manufacturing, facilitating real-time monitoring and optimization across both upstream and downstream processing stages. Raman spectroscopy has often been used for monitoring metabolic substrates and waste products, such as glucose and lactate. Using Raman spectroscopy for the continuous monitoring of critical parameters such as glucose and lactate levels has been extensively demonstrated in literature and has traditionally used several batches to develop the chemometric models needed for monitoring. Tornado Spectral Systems – A Bruker Company, aims to demonstrate the integration of plug-and-play and semi-plug-and-play models for their efficacy in minimizing offline data requirements and accelerating model development, thereby enhancing process control and efficiency.
Raman spectroscopy also has great applicability in downstream processing, where the molecular specificity of Raman signals enables the real-time monitoring of individual proteins and other materials. This specificity proves instrumental in affinity chromatography, where precise protein purification protocols are essential for optimizing bioprocess efficiency. Raman has been traditionally limited in its applications in BioPharma downstream due to slow measurement times. The High Throughput Virtual SlitTM (HTVSTM) technology allows for more than 10x greater signal throughput, enabling Raman to be more effectively applied in the downstream space. Experimental data on Raman monitoring of breakthrough detection in affinity chromatography is demonstrated, showing the ability of Raman to track a target protein (Human Serum Albumin) in the presence of confounding proteins (lysozyme).
Through its ability to provide real-time, molecular-level insights, Raman spectroscopy facilitates the advancement of bioprocess monitoring and optimization, ultimately contributing to the production of high-quality biopharmaceuticals.
Shaun Fraser – Lead Scientist, Tornado – a Bruker Company
This complimentary symposium is brought to you by ProAnalytics, Aber Instruments, Securecell and Tornado (a Bruker Company).
May 16, 2024 from 1pm–5pm ET at the Residence Inn at Boston Cambridge
